Journal: Life Science Alliance

Article Title: Macrophage Sult2b1 promotes pathological neovascularization in age-related macular degeneration

doi: 10.26508/lsa.202302020

Figure Lengend Snippet: (A, B) Representative images of double staining for F4/80 (green) and YM1 (red) (A) and quantification (B) of YM1-positive macrophages in CNV lesions of WT and Sult2b1 -/- mice (n = 6 per group). (C, D) Representative images of double staining for F4/80 (green) and ARG1 (red) (C) and quantification (D) of ARG1-positive macrophages in CNV lesions of WT and Sult2b1 -/- mice (n = 6 per group). Blue indicates DAPI. INL, inner nuclear layer; ONL, outer nuclear layer; RPE, retinal pigment epithelium. (E) Western blot analysis of SULT2B1 in WT BMDMs treated with or without IL-4. GAPDH was used as a loading control. (F) Western blot analysis of YM1 and ARG1 in BMDMs from WT and Sult2b1 -/- mice treated with or without IL-4. β-Actin was used as a loading control. (G) Representative images of tube formation by C166 cells cultured with conditioned medium from WT or Sult2b1 -/- M2 BMDMs. Scale bar, 100 μm. (H) Quantification of tube formation by C166 cells (n = 5). (I) Representative images of tube formation by HUVECs cultured with a conditioned medium from WT or Sult2b1 -/- M2 BMDMs. Scale bar, 100 μm. (I, J) Quantification of tube formation by HUVECs (I) (n = 5). Data information: in (B, D, H, J), data are presented as the mean ± SD. ** P < 0.01, *** P < 0.001, **** P < 0.0001 (unpaired two-tailed t test). Source data are available for this figure.

Article Snippet: After blocking with 5% nonfat dry milk in TBST (TBS containing Tween-20) for 1 h at room temperature, the membranes were incubated at 4°C overnight with primary antibodies against GAPDH (10494-1-AP, 1:1,000; Proteintech), β-actin (3779, 1:1,000; Prosci), YM1 (1404, 1:1,000; Stem Cell), ARG1 (16001-1-AP, 1:5,000; Proteintech), SULT2B1 (ab254616, 1:1,000; Abcam), LXRα (ab176323, 1:1,000; Abcam), LXRβ (ab28479, 1:1,000; Abcam), ABCA1 (ab18180, 1:500; Abcam) or ABCG1 (ab52617, 1:1,000; Abcam).

Techniques: Double Staining, Western Blot, Control, Cell Culture, Two Tailed Test

Journal: Journal of Drug Delivery Science and Technology

Article Title: Nanoparticle-based Olaparib delivery enhances its effect, and improves drug sensitivity to cisplatin in triple negative breast cancer

doi: 10.1016/j.jddst.2022.103731

Figure Lengend Snippet: Fig. 6. Western blot assay. The expression levels of Bax and Bcl-2 protein were detected by Western blot in MDA-MB-231 cells and MDA-MB-231/CDDP cells. The GAPDH was used as internal reference.

Article Snippet: After blocking with 5% skim milk for 2 h at 25 ◦C, the sheet was incubated overnight at 4 ◦C with primary antibodies against GAPDH (Origene, USA), Bax (GeneTex, USA), and Bcl-2 (GeneTex, USA).

Techniques: Western Blot, Expressing